Customized E2 Screening Kit

The E2 Screening Kit is developed for identification of the E2 Ub-conjugating enzyme(s) for a specific E3 Ub ligase and/or a protein substrate. N/A

Additional Information

Product Name: Customized E2 Screening Kit
Also Known As: N/A
Catalog No.: J1200
Size: 1 Kit
Molecular Weight: N/A
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: 90% - 95% based on SDS-PAGE
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF datasheet, MSDS
NCBI RefSeq: N/A
Image(s): No
Shipping Method: Dry ice shipping
References: N/A

Details

* When place an order, please select any ten (10) E2s from the list on the right side of this page.

Description

The E2 Screening Kit is developed for identification of the E2 Ub-conjugating enzyme(s) for a specific E3 Ub ligase and/or a protein substrate.

Components

• 20X UBE1 (2 µM).........................................................50µl
• 10X E2 Ub conjugating enzymes
(pick 10 E2s from the list below, 20µM)......................20 µl each
• 10X Human Ubiquitin (500 µM).................................100 µl
• 10X Ubiquitination Buffer...........................................500 µl
• 40 mM ATP................................................................125 µl

E2 Name

1: 6XHis-Ube2A (Rad6A)
2: 6XHis-Ube2B
3: 6XHis-Ube2C(UbcH10 )
4: 6XHis-Ube2D1 (UbcH5A)
5: 6XHis-Ube2D2 (UbcH5B)
6: 6XHis-Ube2D3 (UbcH5C)
7: 6XHis-Ube2D4 (UbcH5D)
8: 6XHis-Ube2E1 (UbcH6)
9: 6XHis-Ube2E2 (UbcH8)
10: 6XHis-Ube2E3 (UbcH9)
11: 6XHis-Ube2F
12: 6XHis-Ube2G1
13: 6XHis-Ube2G2
14: 6XHis-Ube2H (UbcH2)
15: 6XHis-Ube2I
16: 6XHis-Ube2J1(1-282)
17: GST-Ube2K (E2-25K)
18: 6XHis-Ube2L3
19: 6XHis-Ube2L6
20: 6XHis-Ube2M (UbcH12)
21: 6XHis-UbeN (Ubc13/Uev1a)
22: 6XHis-Ube2Q2
23: 6XHis-Ube2R1 (Cdc34)
24: 6XHis-Ube2R2
25: 6XHis-Ube2S
26: 6XHis-Ube2T
27: 6xHis-Ubc13/UbE2V2
28: 6XHis-Ube2W
29: 6XHis-Ube2Z