Additional Information
Product Name: | Ub-Rhodamine 110 |
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Also Known As: | N/A |
Catalog No.: | M3020 |
Size: | 50 µg |
Molecular Weight: | 8936 Da by MS |
Species: | Human |
Source: | Synthetic |
Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
Concentration: | N/A |
Quality Assurance: | ≥95% by RP-HPLC and SDS-PAGE |
Storage: | Powder at −20°C; solution at −80°C. Avoid multiple freeze/thaw cycles. |
PDF Data Sheet: | PDF Datasheet, MSDS |
NCBI RefSeq: | N/A |
Image(s): | (Click image to enlarge) |
Shipping Method: | Dry ice shipping |
References: | 1. A. Tirat et al., (2005) Anal. Biochem., 343, 244-255. 2. U. Hassiepin et al., (2007) Anal. Biochem., 371, 201-207. |
Details
Ub-Rhodamine 110 is a quenched, fluorescent substrate for deubiquitinating enzymes. Cleavage of the amide bond between the C-terminal glycine of ubiquitin and rhodamine110 results in an increase in rhodamine fluorescence at 535 nm (Exc. 485 nm).
Sample Preparation:
Dilute stock solution into required buffer. Typical reaction concentrations vary from 0.1 - 1 µM Images: (Click image to enlarge)
Sample Preparation:
Dilute stock solution into required buffer. Typical reaction concentrations vary from 0.1 - 1 µM Images: (Click image to enlarge)