UbE3A (E6AP)

UbE3A (also called E6AP) is a 100 kDa HECT (homologous to E6AP carboxyl terminus) domain ubiquitin ligase containing two E2 binding sites for UbcH7 and UbcH8. UbE3A was found to bind to the human papillomavirus (HPV) oncoprotein E6 and mediate ubiquitination of p53. A frameshift mutation in the UbE3A gene leads to loss of function and causes Angelman Syndrome.

Additional Information

Product Name: UbE3A (E6AP)
Also Known As: AS; ANCR; E6-AP; E6AP; HPVE6A; EPVE6AP
Catalog No.: K1411
Size: 50 µg
Molecular Weight: 100.7 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~90% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF Datasheet
NCBI RefSeq: NM_000462.3
Image(s): Please click product PDF datasheet above to view a representative SDS-PAGE of UbE3A.
Shipping Method: Dry ice shipping
References: 1. Scheffner M, et al. (1990) Cell 63, 1129­1136.
2. Scheffner M, et al. (1993) Cell 75, 495­505.
3. Abaied L, et al. (2009) Am J Med Genet Part A 152A, 141­146.
4. Ronchi VP, et al. (2013) JBC 288, 10349­10360.

Details

UbE3A (also called E6AP) is a 100 kDa HECT (homologous to E6AP carboxyl terminus) domain ubiquitin ligase containing two E2 binding sites for UbcH7 and UbcH8. UbE3A was found to bind to the human papillomavirus (HPV) oncoprotein E6 and mediate ubiquitination of p53. A frameshift mutation in the UbE3A gene leads to loss of function and causes Angelman Syndrome. This product is made from GST-UbE3A (catalog # K1410) by removal of the N-terminal GST tag.