Additional Information
| Product Name: | GST-Ube2K (E2-25K) | ||
|---|---|---|---|
| Also Known As: | LIG; HIP2; HYPG; UBC1; E2-25K; DKFZp564C1216; DKFZp686J24237 | ||
| Catalog No.: | C2800 | ||
| Size: | 25 µg | ||
| Molecular Weight: | 22.4 kDa | ||
| Species: | Human | ||
| Source: | Bacterial recombinant | ||
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol | ||
| Concentration: | See tube label | ||
| Quality Assurance: | ~90% by SDS-PAGE, see datasheet for gel image | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||
| NCBI RefSeq: | NM_005339 | ||
| Image(s): | (Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | 1. Kalchman MA, et al. (1996) J Biol Chem 271, 19385 – 19394. 2. Haldeman MT, et al. (1997) Biochemistry 36(34), 10526 – 10537. 3. Yao T, et al. (2000) J Biol Chem 275, 36862–36868. |
Details
Ube2K (or E2-25K) is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. It contains a C-terminal UBA domain and an E2 catalytic domain. Ube2K contains a 47 amino acid residue tail that contributes greatly to its specific characteristics. Ube2K is one of the E2s that are capable of forming K48-linked polyubiquitin chains.
Images:
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified GST-Ube2K (E2-25K) |
