6xHis-Ube2W

Ube2W is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. It has been shown to catalyze monoubiquitination of Fanconi anemia complementation group L (FANCL), an E3 Ub ligase. The FANCL is part of a complex that is able to monoubiquitinate FANCD2 during DNA repair. Ube2W can also interact with the RING domain of the breast and ovarian cancer susceptibility protein called BRCA1.

Additional Information

Product Name: 6xHis-Ube2W
Also Known As: UBC16; UBC-16; hUBC-16; FLJ11011
Catalog No.: C4001
Size: 100 µg
Molecular Weight: 18.7 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~90% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_001001481
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2W
Shipping Method: Dry ice shipping
References: 1. Yin G, et al. (2006) Front Biosci 11, 1500 – 1507.
2. Christensen DE, et al. (2007) Nat Struct Mol Biol 14, 941 – 948.
3. Alpi AF, et al. (2008) Mol Cell 32, 767 – 777.
4. Zhang Y, et al. (2011) Mol Cells 31(2), 113 – 122.

Details

Ube2W is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. It has been shown to catalyze monoubiquitination of Fanconi anemia complementation group L (FANCL), an E3 Ub ligase. The FANCL is part of a complex that is able to monoubiquitinate FANCD2 during DNA repair. Ube2W can also interact with the RING domain of the breast and ovarian cancer susceptibility protein called BRCA1.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2W