6xHis-Ube2V2

Ube2V2 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2V2 is the human homolog of yeast Mms2 and shares 50% amino acid sequence identity. Its main function is to be used in DNA repair. Ube2V2 forms a heterodimer with the human homolog of Ubc13 (an E2). This complex promotes formation of K63-linked polyUb chains.

Additional Information

Product Name: 6xHis-Ube2V2
Also Known As: MMS2; UEV2; EDPF1; UEV-2; DDVIT1; EDAF-1; EDPF-1; DDVit-1
Catalog No.: C3900
Size: 25 µg
Molecular Weight: 16.4 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_003350
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2V2
Shipping Method: Dry ice shipping
References: 1. Fritsche J, et al. (1997) Biochem Biophys Res Commun 235(2), 407-12.
2. Xiao W, et al. (1998) Nuclei Acids Res 26(17), 3908 – 3914.
3. McKenna S, et al. (2001) J Biol Chem 276(43), 40120 – 40126.

Details

Ube2V2 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2V2 is the human homolog of yeast Mms2 and shares 50% amino acid sequence identity. Its main function is to be used in DNA repair. Ube2V2 forms a heterodimer with the human homolog of Ubc13 (an E2). This complex promotes formation of K63-linked polyUb chains.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2V2