6xHis-Ube2T

Ube2T is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2T interacts with the E3 ligase called Fanconi anemia complementation group L (FANCL) that is part of a larger complex to regulate DNA damage repair. Once FANCL interacts with Ube2T, the complex is then able to monoubiquitinate FANCD2 that leads to the DNA repair. Ube2T can also interact with the BRCA1/BARD1 complex.

Additional Information

Product Name: 6xHis-Ube2T
Also Known As: PIG50; HSPC150
Catalog No.: C3800
Size: 25 µg
Molecular Weight: 22.5 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~90% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_014176
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2T
Shipping Method: Dry ice shipping
References: 1. Zhang QH, et al. (2000) Genome Res 10, 1546 – 1560.
2. Machida YJ, et al. (2006) Mol Cell 23, 589-96.
3. Hao J, et al. (2008) Tumour Biol 29, 195 – 203.
4. Ueki T, et al. (2009) Cancer Res 69(22), 8752 – 8760.

Details

Ube2T is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2T interacts with the E3 ligase called Fanconi anemia complementation group L (FANCL) that is part of a larger complex to regulate DNA damage repair. Once FANCL interacts with Ube2T, the complex is then able to monoubiquitinate FANCD2 that leads to the DNA repair. Ube2T can also interact with the BRCA1/BARD1 complex.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2T