6xHis-Ube2S

Ube2S is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2S works with a Ub ligase, anaphase-promoting complex (APC/C), to ubiquitinate proteins like Cyclin B1 to be degraded by the 26S proteasome. Ube2S was found to produce K11-specific Ub chains.

Additional Information

Product Name: 6xHis-Ube2S
Also Known As: EPF5; E2EPF; E2-EPF
Catalog No.: C3700
Size: 25 µg
Molecular Weight: 23.8 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~90% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_014501
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2S
Shipping Method: Dry ice shipping
References: 1. Liu Z, et al. (1992) J Biol Chem 267(22), 15829 – 15835.
2. Garnett MJ, et al. (2009) Nat Cell Biol 11, 1363 – 1369.
3. Williamson A, et al. (2009) Proc Natl Acad Sci USA 106, 18213 – 18218.
4. Wu T, et al. (2010) Proc Natl Acad Sci USA 107(4), 1355 – 1360.

Details

Ube2S is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2S works with a Ub ligase, anaphase-promoting complex (APC/C), to ubiquitinate proteins like Cyclin B1 to be degraded by the 26S proteasome. Ube2S was found to produce K11-specific Ub chains.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2S