6xHis-UbE2S(1-156)

UbE2S is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. UbE2S works with the Ub ligase, anaphase-promoting complex (APC/C), to ubiquitinate proteins like Cyclin B1. UbE2S was found to mediate synthesis of K11-specific polyubiquitin chains. UbE2S(1-156) lacks the C-terminal region of UbE2S, and this truncated version is more efficient than UbE2S in catalyzing synthesis of K11-linked polyubiquitin chains.

Additional Information

Product Name: 6xHis-UbE2S(1-156)
Also Known As: EPF5; E2EPF; E2-EPF
Catalog No.: C3710
Size: 25 µg
Molecular Weight: 17.4 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_014501.2
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-UbE2S(1-156)
Shipping Method: Dry ice shipping
References: N/A

Details

UbE2S is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. UbE2S works with the Ub ligase, anaphase-promoting complex (APC/C), to ubiquitinate proteins like Cyclin B1. UbE2S was found to mediate synthesis of K11-specific polyubiquitin chains. UbE2S(1-156) lacks the C-terminal region of UbE2S, and this truncated version is more efficient than UbE2S in catalyzing synthesis of K11-linked polyubiquitin chains.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-UbE2S(1-156)