6xHis-Ube2R2

Ube2R2 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2R2 is phosphorylated by CK2 before it can bind to SCF (Skp1, Cul1, F-box) subunit β-TrCP. This E2 catalyzes K48 – linked Ub chains, which when conjugated to substrates, are targeted to the 26S proteasome for degradation.

Additional Information

Product Name: 6xHis-Ube2R2
Also Known As: UBC3B; CDC34B; E2-CDC34B; FLJ20419; MGC10481
Catalog No.: C3601
Size: 100 µg
Molecular Weight: 27.2 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~90% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_017811
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2R2
Shipping Method: Dry ice shipping
References: 1. Semplici F, et al. (2002) Oncogene 21, 3978 – 3987.
2. Jin J, et al. (2007) Nature 447(7148), 1135 – 1138.
3. David Y, et al. (2010) J Biol Chem 285(12), 8595 – 8604.

Details

Ube2R2 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2R2 is phosphorylated by CK2 before it can bind to SCF (Skp1, Cul1, F-box) subunit β-TrCP. This E2 catalyzes K48 – linked Ub chains, which when conjugated to substrates, are targeted to the 26S proteasome for degradation.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2R2