6xHis-Ube2R1 (UbcH3, Cdc34)

Ube2R1 (UbcH3) is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2R1 was first discovered during screening for cell cycle regulatory mutations that occur in yeast. While Ube2R1 is able to create diubiquitin chains by itself, it works far more efficiently and effectively with the E3 ligase Skp1 (S- phase kinase- associated protein 1)/cullin/F-box, also known as SCF, to promote substrate polyubiquitination.

Additional Information

Product Name: 6xHis-Ube2R1 (UbcH3, Cdc34)
Also Known As: UBC3; UBCH3; UBE2R1; E2-CDC34
Catalog No.: C3501
Size: 100 µg
Molecular Weight: 26.7 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~90% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_004359
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2R1 (UbcH3, Cdc34)
Shipping Method: Dry ice shipping
References: 1. Plon SE, et al. (1993) Proc Natl Acad Sci USA 90(22), 10484 – 10488.
2. Gazdoiu S, et al. (2005) Proc Natl Acad Sci USA 102(42), 15053 – 15058.
3. Sadowski M, et al. (2007) Biochem J 405, 569 – 581.
4. Butz N, et al. (2005) Exp Cell Res 303(2), 482 – 493.

Details

Ube2R1 (UbcH3) is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2R1 was first discovered during screening for cell cycle regulatory mutations that occur in yeast. While Ube2R1 is able to create diubiquitin chains by itself, it works far more efficiently and effectively with the E3 ligase Skp1 (S- phase kinase- associated protein 1)/cullin/F-box, also known as SCF, to promote substrate polyubiquitination.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2R1 (UbcH3, Cdc34)