Additional Information
| Product Name: | 6xHis-Ube2R1 (UbcH3, Cdc34) | ||
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| Also Known As: | UBC3; UBCH3; UBE2R1; E2-CDC34 | ||
| Catalog No.: | C3501 | ||
| Size: | 100 µg | ||
| Molecular Weight: | 26.7 kDa | ||
| Species: | Human | ||
| Source: | Bacterial recombinant | ||
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol | ||
| Concentration: | See tube label | ||
| Quality Assurance: | ~90% by SDS-PAGE, see datasheet for gel image | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||
| NCBI RefSeq: | NM_004359 | ||
| Image(s): | (Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | 1. Plon SE, et al. (1993) Proc Natl Acad Sci USA 90(22), 10484 – 10488. 2. Gazdoiu S, et al. (2005) Proc Natl Acad Sci USA 102(42), 15053 – 15058. 3. Sadowski M, et al. (2007) Biochem J 405, 569 – 581. 4. Butz N, et al. (2005) Exp Cell Res 303(2), 482 – 493. |
Details
Ube2R1 (UbcH3) is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2R1 was first discovered during screening for cell cycle regulatory mutations that occur in yeast. While Ube2R1 is able to create diubiquitin chains by itself, it works far more efficiently and effectively with the E3 ligase Skp1 (S- phase kinase- associated protein 1)/cullin/F-box, also known as SCF, to promote substrate polyubiquitination.
Images:
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-Ube2R1 (UbcH3, Cdc34) |
