6xHis-Ube2N (6xHis-Ubc13/Uev1a)

Ube2N (UbcH13/Uev1a) is a complex that acts as an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Uev1a must interact with Ubc13 because it lacks the active site that other E2s posses. Ube2N has been known to catalyze K63 – linked Ub chains which aides in activation of kinases in NF – kappaB signaling pathway, amongst other tasks.

Additional Information

Product Name: 6xHis-Ube2N (6xHis-Ubc13/Uev1a)
Also Known As: Ubc13 (UbcH-ben; MGC8489; MGC131857); Uev1a (Ube2V1; CIR1; UEV1; CROC1; UBE2V; UEV-1; CROC-1)
Catalog No.: C3200
Size: 25 µg
Molecular Weight: Ubc13 (17.1 kDa), Uev1a (19.3 kDa)
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_003348 (Ubc13); NM_021988 (Uev1a)
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2N (6xHis-Ubc13/Uev1a)
Shipping Method: Dry ice shipping
References: 1. Yamaguchi T, et al. (1996 J Biochem 120(3), 494 – 497.
2. Hofmann RM, et al. (1999) Cell 96(5), 645-653.
3. Ashley C, et al. (2002) Gene 285(1 – 2), 183 – 191.
4. Andersen PL, et al. (2005) J Cell Biol 170(5), 745 – 755.

Details

Ube2N (UbcH13/Uev1a) is a complex that acts as an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Uev1a must interact with Ubc13 because it lacks the active site that other E2s posses. Ube2N has been known to catalyze K63 – linked Ub chains which aides in activation of kinases in NF – kappaB signaling pathway, amongst other tasks.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2N (6xHis-Ubc13/Uev1a)