6xHis-Ube2L6

Ube2L6 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. One function of this E2 is that it is involved in ubiquitination and degradation of a neuron – specific protein called syntaxin 1. Recently Ube2L6 has been shown to work with the Isg15 E1, Ube1L, to conjugate Isg15 to substrates as well.

Additional Information

Product Name: 6xHis-Ube2L6
Also Known As: RIG-B; UBCH8; MGC40331
Catalog No.: C3001
Size: 100 µg
Molecular Weight: 17.8 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_004223
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2L6
Shipping Method: Dry ice shipping
References: 1. Kumar S, et al. (1997) J Biol Chem 272, 13548-54.
2. Chin LS, et al. (2002) J Biol Chem 277, 35071 – 35079.
3. Zhao C, et al. (2004) Proc Natl Acad Sci 101(20), 7578 – 7582.
4. Desai SD, et al. (2006) Cancer Res 66(2), 921 – 928.
5. Durfee LA, et al. (2008) J Biol Chem 283(35), 23895 – 23902.

Details

Ube2L6 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. One function of this E2 is that it is involved in ubiquitination and degradation of a neuron – specific protein called syntaxin 1. Recently Ube2L6 has been shown to work with the Isg15 E1, Ube1L, to conjugate Isg15 to substrates as well.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2L6