6xHis-Ube2L3

Ube2L3 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2L3 is potentially a regulator of the S phase of the cell cycle. The Ube2L3 levels decrease during the S phase, but then are increased during the G2 phase.

Additional Information

Product Name: 6xHis-Ube2L3
Also Known As: E2-F1; L-UBC; UBCH7; UbcM4
Catalog No.: C2900
Size: 25 µg
Molecular Weight: 17.9 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~90% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_001256355
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2L3
Shipping Method: Dry ice shipping
References: 1. Moynihan TP, et al. (1996) Mamm Genome 7(7), 520 – 525.
2. Ardley HC, et al. (2000) Biochim Biophys Acta 1491(1 – 3), 57 – 64.
3. Whitcomb EA, et al. (2009) Cell Div 4, 17.
4. Shimura H,et al. (2001) Science 293, 263 – 269.

Details

Ube2L3 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2L3 is potentially a regulator of the S phase of the cell cycle. The Ube2L3 levels decrease during the S phase, but then are increased during the G2 phase.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2L3