6xHis-Ube2J1(1-282)

UbE2J1 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. UBE2J1 is a membrane protein localized on the endoplasmic reticulum (ER), where it catalyzes ubiquitination of misfolded membrane proteins with other ubiquitination components. UbE2J1(1-282) lacks the C-terminal membrane-localization region, but still possesses Ub conjugating activity.

Additional Information

Product Name: 6xHis-Ube2J1(1-282)
Also Known As: UBC6; UBC6E; Ubc6p; CGI-76; NCUBE1; HSPC153; HSPC205; NCUBE-1; HSU93243
Catalog No.: C2600
Size: 25 µg
Molecular Weight: 31.2 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~80% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2J1(1-282)
Shipping Method: Dry ice shipping
References: 1. Lester D, et al. (2000) Biochem Biophys Res Commun 269, 474 – 480.
2. Tiwari S, et al. (2001) J Biol Chem 276, 16193 – 16200.
3. Lenk U, et al. (2002) J Cell Sci 115(Pt 14), 3007 – 3014.
4. Wang X, et al. (2009) J Cell Biol 187(5), 655 – 668.

Details

UbE2J1 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. UBE2J1 is a membrane protein localized on the endoplasmic reticulum (ER), where it catalyzes ubiquitination of misfolded membrane proteins with other ubiquitination components. UbE2J1(1-282) lacks the C-terminal membrane-localization region, but still possesses Ub conjugating activity.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2J1(1-282)