Additional Information
| Product Name: | 6xHis-Ube2I | ||
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| Also Known As: | P18; UBC9; C358B7.1 | ||
| Catalog No.: | C2500 | ||
| Size: | 25 µg | ||
| Molecular Weight: | 18 kDa | ||
| Species: | Human | ||
| Source: | Bacterial recombinant | ||
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol | ||
| Concentration: | See tube label | ||
| Quality Assurance: | ~95% by SDS-PAGE, see datasheet for gel image | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||
| NCBI RefSeq: | NM_003345 | ||
| Image(s): | (Click image to enlarge)
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| Shipping Method: | FedEx | ||
| References: | 1. Wang ZY, et al. (1996) J Biol Chem 271(40), 24811 – 24816. 2. Golebiowski F, et al. (2004) Arch Biochem Biophys 428(2), 160 – 164. 3. Johnson ES, (2004) Rev. Biochem. 73:355–382. 4. Reverter D, et al. (2005) Nature 435(7042), 687 – 692. |
Details
Ube2I (Ubc9) is an E2 enzyme in the E1, E2, and E3 cascade to link SUMO to a substrate. This E2 shares a similar sequence and structure to the Ub E2s and is the only known SUMO conjugating enzyme at this time. Ube2I shares 56% amino acid identity with the yeast homolog called Ub – conjugating enzyme 9. While a hypothesis has been proposed that Ube2I may not need an E3 to ligate SUMO.
Images:
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-Ube2I |
