6xHis-Ube2I

Ube2I (Ubc9) is an E2 enzyme in the E1, E2, and E3 cascade to link SUMO to a substrate. This E2 shares a similar sequence and structure to the Ub E2s and is the only known SUMO conjugating enzyme at this time. Ube2I shares 56% amino acid identity with the yeast homolog called Ub – conjugating enzyme 9. While a hypothesis has been proposed that Ube2I may not need an E3 to ligate SUMO.

Additional Information

Product Name: 6xHis-Ube2I
Also Known As: P18; UBC9; C358B7.1
Catalog No.: C2501
Size: 100 µg
Molecular Weight: 18 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_003345
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2I
Shipping Method: Dry ice shipping
References: 1. Wang ZY, et al. (1996) J Biol Chem 271(40), 24811 – 24816.
2. Golebiowski F, et al. (2004) Arch Biochem Biophys 428(2), 160 – 164.
3. Johnson ES, (2004) Rev. Biochem. 73:355–382.
4. Reverter D, et al. (2005) Nature 435(7042), 687 – 692.

Details

Ube2I (Ubc9) is an E2 enzyme in the E1, E2, and E3 cascade to link SUMO to a substrate. This E2 shares a similar sequence and structure to the Ub E2s and is the only known SUMO conjugating enzyme at this time. Ube2I shares 56% amino acid identity with the yeast homolog called Ub – conjugating enzyme 9. While a hypothesis has been proposed that Ube2I may not need an E3 to ligate SUMO.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2I