6xHis-Ube2H (UbcH2)

Ube2H is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2H is the human homolog to its yeast counterpart, Ubc8. Ube2H ubiquitinates histone H2A in vitro without the assistance of any E3 ligase. This E2 could also play a role in the development autism disorder because of its location on the 7q32 chromosomal region.

Additional Information

Product Name: 6xHis-Ube2H (UbcH2)
Also Known As: UBC8; UBCH; UBCH2; E2-20K
Catalog No.: C2400
Size: 25 µg
Molecular Weight: 20.7 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_003344
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2H (UbcH2)
Shipping Method: Dry ice shipping
References: 1. Kaiser P, et al. (1994) J Biol Chem 269, 8797 – 8802.
2. Kaiser P, et al. (1995) FEBS Lett 377, 193 – 196.
3. Vourc’h P, et al. (2003) Psychiatr Genet 13, 221 – 225.
4. Li YP, et al. (2003) FASEB J 17(9), 1048 – 1057.

Details

Ube2H is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2H is the human homolog to its yeast counterpart, Ubc8. Ube2H ubiquitinates histone H2A in vitro without the assistance of any E3 ligase. This E2 could also play a role in the development autism disorder because of its location on the 7q32 chromosomal region.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2H (UbcH2)