Additional Information
| Product Name: | 6xHis-UbE2E3 (UbcH9) | ||
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| Also Known As: | UBCH9; UbcM2 | ||
| Catalog No.: | C2000 | ||
| Size: | 25 µg | ||
| Molecular Weight: | 22.9 kDa | ||
| Species: | Human | ||
| Source: | Bacterial recombinant | ||
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol | ||
| Concentration: | See tube label | ||
| Quality Assurance: | ~95% by SDS-PAGE, see datasheet for gel image | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||
| NCBI RefSeq: | NM_006357 | ||
| Image(s): | (Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | 1. Ito K, et al. (1999) Cyotgenet Cell Genet 84 (1 – 2), 99 – 104. 2. Ito K, et al. (2001) Eur J Biochem 268, 2725–2732. 3. Debonneville C, et al. (2004) Mol Cell Biol 24, 2397–2409. 4. Plafker KS, et al. (2008) Invest Ophthalmol Vis Sci 49(12), 5611 – 5618. |
Details
UbE2E3 (also known as UbcH9) is an E2 enzyme, which is part of the E1, E2, E3 cascade that is responsible for ubiquitination of protein substrates. It has been found to interact with the E3 enzyme Nedd4-2 in order to regulate ubiquitination of the epithelial Na+ channel (ENaC). Studies have shown that UbE2E3 may be able to associate with the substrate directly, which implies the assistance of an E3 may not be required under all circumstances.
Images:
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-Ube2E3 (UbcH9) |
