6xHis-Ube2E3 (UbcH9)

Ube2E3 (also known as UbcH9) is an E2 enzyme, which is part of the E1, E2, E3 cascade that is responsible for ubiquitination of protein substrates. It has been found to interact with the E3 enzyme Nedd4-2 in order to regulate ubiquitination of the epithelial Na+ channel (ENaC). Studies have shown that Ube2E3 may be able to associate with the substrate directly, which implies the assistance of an E3 may not be required under all circumstances.

Additional Information

Product Name: 6xHis-Ube2E3 (UbcH9)
Also Known As: UBCH9; UbcM2
Catalog No.: C2001
Size: 100 µg
Molecular Weight: 22.9 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_006357
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2E3 (UbcH9)
Shipping Method: Dry ice shipping
References: 1. Ito K, et al. (1999) Cyotgenet Cell Genet 84 (1 – 2), 99 – 104.
2. Ito K, et al. (2001) Eur J Biochem 268, 2725–2732.
3. Debonneville C, et al. (2004) Mol Cell Biol 24, 2397–2409.
4. Plafker KS, et al. (2008) Invest Ophthalmol Vis Sci 49(12), 5611 – 5618.

Details

Ube2E3 (also known as UbcH9) is an E2 enzyme, which is part of the E1, E2, E3 cascade that is responsible for ubiquitination of protein substrates. It has been found to interact with the E3 enzyme Nedd4-2 in order to regulate ubiquitination of the epithelial Na+ channel (ENaC). Studies have shown that Ube2E3 may be able to associate with the substrate directly, which implies the assistance of an E3 may not be required under all circumstances.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2E3 (UbcH9)