Additional Information
| Product Name: | 6xHis-UbE2E2 (UbcH8) | ||
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| Also Known As: | UBCH8 | ||
| Catalog No.: | C1901 | ||
| Size: | 100 µg | ||
| Molecular Weight: | 22.3 kDa | ||
| Species: | Human | ||
| Source: | Bacterial recombinant | ||
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol | ||
| Concentration: | See tube label | ||
| Quality Assurance: | ~90% by SDS-PAGE, see datasheet for gel image | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||
| NCBI RefSeq: | NM_152653 | ||
| Image(s): | (Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | 1. Kimura M, et al. (1997) Cytogenet Cell Genet 78(2), 107 – 111. 2. Zhang Y, et al. (2000) Proc Natl Acad Sci USA 97(24), 13354 – 13359. 3. Ito K, et al. (2001) European Journal of Biochemistry 268, 2725–2732. 4. Lok GTM, et al. (2012) Nucleic Acids Res 40(1), 196-205. |
Details
UbE2E2 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. It shares 96% identical amino acid sequence with UbE2E1 and UbE2E3 with the exception of the N-terminal extension that has little in common with the other two E2s. UbE2E2 interacts with ARA54 and RNF8. The RNF8/ UbE2E2 interaction is found to catalyze K48-linked poly-ubiquitination. UbE2E2 may play a role in ubiquitination and subsequent proteasomal degradation of the E3 Ub ligase Parkin.
Images:
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified6xHis-Ube2E2 (UbcH8) |
