6xHis-Ube2E2 (UbcH8)

Ube2E2 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. It shares 96% identical amino acid sequence with Ube2E1 and Ube2E3 with the exception of the N-terminal extension that has little in common with the other two E2s. Ube2E2 interacts with ARA54 and RNF8. The RNF8/ Ube2E2 interaction is found to catalyze K48-linked poly-ubiquitination. Ube2E2 may play a role in ubiquitination and subsequent proteasomal degradation of the E3 Ub ligase Parkin.

Additional Information

Product Name: 6xHis-Ube2E2 (UbcH8)
Also Known As: UBCH8
Catalog No.: C1901
Size: 100 µg
Molecular Weight: 22.3 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~90% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_152653
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified6xHis-Ube2E2 (UbcH8)
Shipping Method: Dry ice shipping
References: 1. Kimura M, et al. (1997) Cytogenet Cell Genet 78(2), 107 – 111.
2. Zhang Y, et al. (2000) Proc Natl Acad Sci USA 97(24), 13354 – 13359.
3. Ito K, et al. (2001) European Journal of Biochemistry 268, 2725–2732.
4. Lok GTM, et al. (2012) Nucleic Acids Res 40(1), 196-205.

Details

Ube2E2 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. It shares 96% identical amino acid sequence with Ube2E1 and Ube2E3 with the exception of the N-terminal extension that has little in common with the other two E2s. Ube2E2 interacts with ARA54 and RNF8. The RNF8/ Ube2E2 interaction is found to catalyze K48-linked poly-ubiquitination. Ube2E2 may play a role in ubiquitination and subsequent proteasomal degradation of the E3 Ub ligase Parkin.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified6xHis-Ube2E2 (UbcH8)