6xHis-Ube2E1 (UbcH6)

Ube2E1 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2E1 was found to locate in the nucleus. It, along with Ube2D1, is known to complex with the E3 ligase Ro52 to ubiquitinate interferon regulatory transcription factors IRF3 and IRF8. Ube2E1 can also complex with a Ub ligase called RING105 to ubiquitinate a tumor suppressor known as tumor – suppressing subchromosomal transferable fragment cDNA (TSSC5).

Additional Information

Product Name: 6xHis-Ube2E1 (UbcH6)
Also Known As: UBCH6
Catalog No.: C1801
Size: 100 µg
Molecular Weight: 21.4 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~90% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_003341
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2E1 (UbcH6)
Shipping Method: Dry ice shipping
References: 1. Nuber U, et al. (1996) J Biol Chem 271(5), 2795 – 2800.
2. Yamada HY, et al. (2006) Oncogene 25(9), 1330 – 1339.
3. Espinosa A, et al. (2008) Exp Cell Res 314, 3605–3613.
4. Espinosa A, et al. (2009) J Exp Med 206, 1661–1671.

Details

Ube2E1 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2E1 was found to locate in the nucleus. It, along with Ube2D1, is known to complex with the E3 ligase Ro52 to ubiquitinate interferon regulatory transcription factors IRF3 and IRF8. Ube2E1 can also complex with a Ub ligase called RING105 to ubiquitinate a tumor suppressor known as tumor – suppressing subchromosomal transferable fragment cDNA (TSSC5).

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2E1 (UbcH6)