6xHis-Ube2D4 (UbcH5D)

Ube2D4 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2D4 has been linked to cadminum toxicity. It might also play a role in LDL cholesterol receptor ubiquitination in an Idol-dependent fashion.

Additional Information

Product Name: 6xHis-Ube2D4 (UbcH5D)
Also Known As: HBUCE1
Catalog No.: C1701
Size: 100 µg
Molecular Weight: 16.6 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_015983
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2D4 (UbcH5D)
Shipping Method: Dry ice shipping
References: 1. Colland F, et al. (2004 Genome Res 14(7), 1324 – 1332.
2. Tokumoto M, et al. (2011) J Toxicol Sci 36(2), 191 – 200.
3. Zhang L, et al. (2011) Genes Dev 25, 1262–1274.

Details

Ube2D4 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2D4 has been linked to cadminum toxicity. It might also play a role in LDL cholesterol receptor ubiquitination in an Idol-dependent fashion.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2D4 (UbcH5D)