6xHis-Ube2D3 (UbcH5C)

Ube2D3 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2D3 is 88% identical to the amino acid sequence of Ube2D1. Ube2D3 works with Mdm2 (an E3 ligase) to ubiquitinate and regulate protein levels of p53 and Mdm2. Ube2D3 has been linked to cadminum toxicity. It might also play a role in LDL cholesterol receptor ubiquitination in an Idol-dependent fashion.

Additional Information

Product Name: 6xHis-Ube2D3 (UbcH5C)
Also Known As: UBC4/5; UBCH5C; E2(17)KB3
Catalog No.: C1600
Size: 25 µg
Molecular Weight: 16.7 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_003340
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2D3 (UbcH5C)
Shipping Method: Dry ice shipping
References: 1. Scheffner M, et al. (1994) Proc Natl Acad Sci USA 91, 8797–8801.
2. Jensen JP, et al. (1995) J Biol Chem 270(51), 30408 – 30414.
3. Saville MK, et al. (2004) J Biol Chem 279, 42169–42181.
4. Tokumoto M, et al. (2011) J Toxicol Sci 36(2), 191 – 200.
5. Zhang L, et al. (2011) Genes Dev 25, 1262–1274.

Details

Ube2D3 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2D3 is 88% identical to the amino acid sequence of Ube2D1. Ube2D3 works with Mdm2 (an E3 ligase) to ubiquitinate and regulate protein levels of p53 and Mdm2. Ube2D3 has been linked to cadminum toxicity. It might also play a role in LDL cholesterol receptor ubiquitination in an Idol-dependent fashion.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2D3 (UbcH5C)