Additional Information
| Product Name: | 6xHis-UbE2D1 (UbcH5A) | ||
|---|---|---|---|
| Also Known As: | USFT; UBCH5; UBC4/5; UBCH5A; E2(17)KB1 | ||
| Catalog No.: | C1401 | ||
| Size: | 100 µg | ||
| Molecular Weight: | 16.6 kDa | ||
| Species: | Human | ||
| Source: | Bacterial recombinant | ||
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol | ||
| Concentration: | See tube label | ||
| Quality Assurance: | ~95% by SDS-PAGE, see datasheet for gel image | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||
| NCBI RefSeq: | NM_003338 | ||
| Image(s): | (Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | 1. Scheffner M, et al. (1994) Proc Natl Acad Sci USA 91, 8797–8801. 2. Huibregtse JM, et al. (1995) Proc Natl Acad Sci USA 92(7), 2563 – 2567. 3. Gehrke SG, et al. (2003) Blood 101, 3288 – 3293. 4. Tokumoto M, et al. (2011) J Toxicol Sci 36(2), 191 – 200. |
Details
UbE2D1 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Two other homologues of UbE2D1 exist called UbE2D2 and UbE2D3. UbE2D2 is 89% identical to the amino acid sequence of UbE2D1 ad UbE2D3 is 88% identical. UbE2D1 has been linked to cadminum toxicity. It might also play a role in LDL cholesterol receptor ubiquitination in an Idol-dependent fashion.
Images:
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-Ube2D1 (UbcH5A) |
