6xHis-Ube2B

Ube2B is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. It shares 95% amino acid sequence identity with Ube2A. One function of Ube2B involves the formation of Ube2B/ Rad18 (an E3 enzyme) complex to monoubiquitinate proliferating cell nuclear antigen (PCNA) thus activating it to repair DNA damage. The Ube2B/Rad18 complex is recruited to the replication fork of the damaged DNA to start the post-replication repair.

Additional Information

Product Name: 6xHis-Ube2B
Also Known As: HR6B; UBC2; HHR6B; RAD6B; E2-17kDa
Catalog No.: C1201
Size: 100 µg
Molecular Weight: 17.3 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_003337
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2B
Shipping Method: Dry ice shipping
References: 1. Jentsch S, et al. (1987) Nature 329(6135), 131 – 134.
2. Koken MH, et al. (1991) Proc Natl Acad Sci USA 88(20), 8865 – 8869.
3. Bailly V, et al. (1994) Genes Dev 8(7), 811 – 820.
4. Tsuji Y, et al. (2008) Genes Cells 13, 343 – 354.

Details

Ube2B is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. It shares 95% amino acid sequence identity with Ube2A. One function of Ube2B involves the formation of Ube2B/ Rad18 (an E3 enzyme) complex to monoubiquitinate proliferating cell nuclear antigen (PCNA) thus activating it to repair DNA damage. The Ube2B/Rad18 complex is recruited to the replication fork of the damaged DNA to start the post-replication repair.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2B