Additional Information
| Product Name: | 6xHis-UbE2A (Rad6A) | ||
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| Also Known As: | UBC2; HHR6A; MRXSN; RAD6A; MRXS30 | ||
| Catalog No.: | C1100 | ||
| Size: | 25 µg | ||
| Molecular Weight: | 17.1 kDa | ||
| Species: | Human | ||
| Source: | Bacterial recombinant | ||
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol | ||
| Concentration: | See tube label | ||
| Quality Assurance: | ~95% by SDS-PAGE, see datasheet for gel image | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||
| NCBI RefSeq: | NM_003336 | ||
| Image(s): | (Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | 1. Jentsch S, et al. (1987) Nature 329(6135), 131 – 134. 2. Koken MH, et al.(1991) Proc Natl Acad Sci USA 88(20), 8865 – 8869. 3. de Leeuw N, et al. (2010) Am J Med Genet A 152A(12), 3084 – 3090. 4. Hibbert RG, et al. (2011) Proc Natl Acad Sci USA 108, 5590–5595. |
Details
UbE2A is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. It shares 95% amino acid sequence identity with UbE2B. One function of UbE2A involves the formation of UbE2A/Rad18 (an E3 enzyme) complex to monoubiquitinate proliferating cell nuclear antigen (PCNA) thus activating it to repair DNA damage. UbE2A is an X – chromosomal gene. Mutations in UbE2A have been linked to intellectual disability (ID), severely impaired speech, amongst other symptoms.
Images:
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-Ube2A (Rad6A) |
