6xHis-Ubc13/UbE2V2

This E2 complex is made of 6xHis-Ubc13 with non-tagged UbE2V2. Ubc13/UbE2V2 is specific for synthesis of lysine 63-linked polyUb chains and plays an important role in regulating DNA damage.

Additional Information

Product Name: 6xHis-Ubc13/UbE2V2
Also Known As: No
Catalog No.: C3910
Size: 25 µg
Molecular Weight: Ubc13 (17.1 kDa), UbE2V2(16.4 kDa)
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_003350
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ubc13/UbE2V2
Shipping Method: Dry ice shipping
References: 1. Fritsche J, et al. (1997) Biochem Biophys Res Commun 235(2), 407-12.
2. Xiao W, et al. (1998) Nuclei Acids Res 26(17), 3908 – 3914.
3. McKenna S, et al. (2001) J Biol Chem 276(43), 40120 – 40126.

Details

This E2 complex is made of 6xHis-Ubc13 with non-tagged UbE2V2. Ubc13/UbE2V2 is specific for synthesis of lysine 63-linked polyUb chains and plays an important role in regulating DNA damage.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ubc13/UbE2V2