6xHis-SAE1/SAE2

SAE1/SAE2 is the heterodimeric E1 enzyme that conjugates SUMO to protein substrates. The conjugation process is very similar to that of protein ubiquitination. SUMO is conjugated onto the catalytic cysteine residue of SAE2 by formation of a thioester bond in an ATP-dependent reaction. The activated SUMO is then passed on to an E2 (Ubc9) to be finally attached to a substrate.

Additional Information

Product Name: 6xHis-SAE1/SAE2
Also Known As: SAE1 (AOS1; SUA1; UBLE1A; HSPC140; FLJ3091); SAE2 (RBBP8; RIM; CTIP; JWDS; SCKL2)
Catalog No.: B1301
Size: 100 µg
Molecular Weight: SAE1 (33 kDa), SAE2 (71.2 kDa)
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_005500 (SAE1); NM_002894 (SAE2)
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-SAE1/SAE2
Shipping Method: Dry ice shipping
References: 1. Desterro JM, et al. (1999) J Biol Chem 274(15), 10618 – 10624.
2. Melchior F (2000) Annu Rev Cell Dev Biol 16, 591 – 626.
3. Schulman BA, et al. (2009) Nat Rev Mol Biol 10(5), 319 – 331.

Details

SAE1/SAE2 is the heterodimeric E1 enzyme that conjugates SUMO to protein substrates. The conjugation process is very similar to that of protein ubiquitination. SUMO is conjugated onto the catalytic cysteine residue of SAE2 by formation of a thioester bond in an ATP-dependent reaction. The activated SUMO is then passed on to an E2 (Ubc9) to be finally attached to a substrate.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-SAE1/SAE2