OTUB1

Deubiquitinating enzymes (DUBs) are proteases that posses the ability to cleave ubiquitin chains or the isopeptide bond that conjugates ubiquitin with a substrate. Human cells have approximately 100 DUBs that play important roles in regulating various cellular events. OTUB1 is a member of the ovarian tumor domain family. It is recognized as a K48 ubiquitin chain-specific DUB and can inhibit E2 enzymes, especially Ubc13 (also known as Ube2N), which is known to synthesize K63-linked ubiquitin chains. OTUB1 may also deubiquitinate TRAF3 and -6. This would negatively regulate virus-triggered type I IFN induction.

Additional Information

Product Name: OTUB1
Also Known As: OTB1; OTU1; HSPC263
Catalog No.: H3000
Size: 25 µg
Molecular Weight: 31.2 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_017670
Image(s): No
Shipping Method: Dry ice shipping
References: 1. Balakirev MY, et al. (2003) EMBO Rep 4(5), 517 – 522.
2. Wang T, et al.(2009) J Mol Biol 386(4), 1011 – 1023.
3. Reyes-Turcu FE, et al. (2009) Annu Rev Biochem 78, 363 – 397.
4. Li S, et al. (2010) J Biol Chem 285(7), 4291 – 4297.
5. Wiener R, et al. (2012) Nature 483(7391), 618 – 622.

Details

Deubiquitinating enzymes (DUBs) are proteases that posses the ability to cleave ubiquitin chains or the isopeptide bond that conjugates ubiquitin with a substrate. Human cells have approximately 100 DUBs that play important roles in regulating various cellular events. OTUB1 is a member of the ovarian tumor domain family. It is recognized as a K48 ubiquitin chain-specific DUB and can inhibit E2 enzymes, especially Ubc13 (also known as Ube2N), which is known to synthesize K63-linked ubiquitin chains. OTUB1 may also deubiquitinate TRAF3 and -6. This would negatively regulate virus-triggered type I IFN induction.