6xHis-SENP8

Neural precursor cell expressed developmentally downregulated 8 (Nedd8) is a ubiquitin-like protein. Nedd8 can be conjugated on culin proteins and this modification plays a key role in activation of culin-based E3 ubiquitin ligases. SENP8 (also called NEDP1) is a cystein protease that specifically cleaves the Nedd8 precursor to generate mature Nedd8 and deconjugates neddylated proteins including culins and p53.

Additional Information

Product Name: 6xHis-SENP8
Also Known As: DEN1; NEDP1; PRSC2
Catalog No.: H9821
Size: 100 µg
Molecular Weight: 24.1 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: > 95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_001166340.1
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-SENP8
Shipping Method: Dry ice shipping
References: 1. Gan-Erdene T, et al. (2003) J Biol Chem. 278(31):28892-900.
2. Mendoza HM, et al. (2003) J Biol Chem. 278(28):25637-43.

Details

Neural precursor cell expressed developmentally downregulated 8 (Nedd8) is a ubiquitin-like protein. Nedd8 can be conjugated on culin proteins and this modification plays a key role in activation of culin-based E3 ubiquitin ligases. SENP8 (also called NEDP1) is a cystein protease that specifically cleaves the Nedd8 precursor to generate mature Nedd8 and deconjugates neddylated proteins including culins and p53.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-SENP8