PLPro

Maturation of SARS-CoV-2 virus requires two proteases, 3C-like protease (3CLP) and Papain-like protease (PLP), to cleave the large polyproteins 1a and 1ab to generate functional proteins. which is essential for genome replication and Coronavirus virion production. Both proteases are encoded by the polyproteins. 3CLP is a cysteine protease and forms a homodimer. 3CLP is the drug target of PF-17321332, a main component of the oral anti-Covid-19 drug Paxlovid. PLP is also a cysteine protease that cleaves polyproteins and has deubiquitinating and deISG15ylating activities. PLP is also a potential anti-Covid-19 drug target.

The recombinant SARS-CoV-2 papain-like protease contains the catalytic domain from Glu746 to Lys1060.

Additional Information

Product Name:

PLPro

Also Known As:

PLPro, PLP, SARSCoV-2 PLP

Catalog No.:

V2121

Size:

1 mg

Molecular Weight:

36 kDa

Species:

SARS-Cov-2

Source:

Bacterial recombinant

Stock:

20 mM Tris, pH7.6 at 4 0C, 150 mM NaCl, 4mM TCEP, 0.01% Tween-20 and 10% Glycerol

Concentration:

See tube label

Quality Assurance:

> 90% purity based on SDS-PAGE.

Storage:

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Data Sheet:

PDF Datasheet， MSDS

NCBI RefSeq:

N/A

Image(s):

(Click image to enlarge)

A. Coomassie stained SDS-PAGE of 5 ug recombinant PLPro.
B. Proteinase activity of recombinant PLPro.
Square: 20µM Dabcyl-FRLKGGAPIKGV-Edans+ 150nM PLPro.
Circle: 20µM Dabcyl-FRLKGGAPIKGV-Edans+ 150nM PLPro + 35µM RGL-0617.
C. Deubiquitinating activity of PLPro.
Square: 0.75µM Ubiquitin Rhodamine 110 + 60nM PLPro
Circle: 0.75µM Ubiquitin Rhodamine 110 + 60nM PLPro + 35µM RGL-0617.

Shipping Method:

Dry ice shipping

References:

Details

Maturation of SARS-CoV-2 virus requires two proteases, 3C-like protease (3CLP) and Papain-like protease (PLP), to cleave the large polyproteins 1a and 1ab to generate functional proteins. which is essential for genome replication and Coronavirus virion production. Both proteases are encoded by the polyproteins. 3CLP is a cysteine protease and forms a homodimer. 3CLP is the drug target of PF-17321332, a main component of the oral anti-Covid-19 drug Paxlovid. PLP is also a cysteine protease that cleaves polyproteins and has deubiquitinating and deISG15ylating activities. PLP is also a potential anti-Covid-19 drug target.
The recombinant SARS-CoV-2 papain-like protease contains the catalytic domain from Glu746 to Lys1060.