Dabcyl-KTSAVLQSGFRKME-Edans

Dabcyl-KTSAVLQSGFRKME-Edans is an internally quenched fluorogenic substrate for the SARS-CoV family 3C-like protease (3CLP, also called main protease or MPro) with a Km value of 17 uM. Upon 3CLP-mediated cleavage between glutamine and serine, the resulting SGFRKME-EDANS fluorescence can be monitored at the excitation/emission wavelengths of 355/538 nm, respectively.

The working concentration is 20-50 µM.

Additional Information

Product Name: Dabcyl-KTSAVLQSGFRKME-Edans
Also Known As: Dabcyl-KTSAVLQSGFRKME-Edans, Dabcyl-KTSAVLQSGFRKME-Edans.TFA, Dabcyl-Lys-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys-Met-Glu-Edans, or Dabcyl-Lys-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys-Met-Glu-Edans trifluoroacetate
Catalog No.: V1010-25
Size: 25 mg
Molecular Weight: 2081.5 Da
Species: N/A
Source: Synthetic
Stock: Powder
Concentration: N/A
Quality Assurance: >95% by HPLC
Storage: Eligible for room temperature shipping. Store at -20°C upon receiving; avoid multiple freeze-thaw cycles after dissolving in DMSO. Protect from light.
PDF Data Sheet: Download PDF datasheet, MSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)

Solid cirlce: 20µM Dabcyl-KTSAVLGSGFRKME-Edans (substrate).
Triangle : Substrate + 100nM GST-3CLPro (catalog# V2010))+ 10µM PF-07321332 (catalog# F1000).
Square: Substrate + 100nM GST-3CLPro.
Shipping Method: Room temperature shipping
References: No

Details

Dabcyl-KTSAVLQSGFRKME-Edans is an internally quenched fluorogenic substrate for the SARS-CoV family 3C-like protease (3CLP, also called main protease or MPro) with a Km value of 17 uM. Upon 3CLP-mediated cleavage between glutamine and serine, the resulting SGFRKME-EDANS fluorescence can be monitored at the excitation/emission wavelengths of 355/538 nm, respectively.
The working concentration is 20-50 µM.