TNFalpha

TNFalpha (Tumor necrosis factor alpha) is produced by immune and epithelial cells. It plays important roles in regulating inflammation, apoptosis, and immune response. It exerts its function mainly by binding of two cell surface receptors TNFR1 and TNFR2. Treating cells with TNFalpha activates the NFkappaB, MAPK, and death signaling pathways. The recombinant TNFalpha protein consists of the extracellular domain (amino acids 77-233) of full length TNFalpha. It induces apoptosis of various cancer cells at the concentration of 10-100 ng/mL + 1 ug/mL cycloheximide (a protein translation inhibitor). It is generated from GST-TNFalpha (catlog # U1130) by removal of the N-terminal GST tag.

Additional Information

Product Name:

TNFalpha

Also Known As:

TNF; DIF; TNFA; TNFSF2; TNF-alpha

Catalog No.:

U1120

Size:

50 µg

Molecular Weight:

25.6 kDa

Species:

Human

Source:

Bacterial recombinant

Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol

Concentration:

See tube label

Quality Assurance:

~ 85% by SDS-PAGE

Storage:

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Data Sheet:

PDF Datasheet

NCBI RefSeq:

NM_000594.3

Image(s):

(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified TNFalpha

Shipping Method:

Dry ice shipping

References:

N/A

Details

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified TNFalpha