GST-TNFalpha

TNFalpha (Tumor necrosis factor alpha) is produced by immune and epithelial cells. It plays important roles in regulating inflammation, apoptosis, and immune response. It exerts its function mainly by binding of two cell surface receptors TNFR1 and TNFR2. Treating cells with TNFalpha activates the NFkappaB, MAPK, and apoptotic signaling pathways. The recombinant GST-TNFalpha protein consists of a N-terminal GT tag with the C-terminal extracellular domain (amino acids 77-233) of full length TNFalpha. It can be used for pulldown assays. It binds TNFR1 and TNFR2 to induce downstream signaling pathways, but often requires higher concentrations than TNFalpha (catalog # U1120).

Additional Information

Product Name: GST-TNFalpha
Also Known As: TNF; DIF; TNFA; TNFSF2; TNF-alpha
Catalog No.: U1130
Size: 50 µg
Molecular Weight: 25.6 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~ 85% by SDS-PAGE
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF Datasheet
NCBI RefSeq: NM_000594.3
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified TNFalpha
Shipping Method: Dry ice shipping
References: N/A

Details

TNFalpha (Tumor necrosis factor alpha) is produced by immune and epithelial cells. It plays important roles in regulating inflammation, apoptosis, and immune response. It exerts its function mainly by binding of two cell surface receptors TNFR1 and TNFR2. Treating cells with TNFalpha activates the NFkappaB, MAPK, and apoptotic signaling pathways. The recombinant GST-TNFalpha protein consists of a N-terminal GT tag with the C-terminal extracellular domain (amino acids 77-233) of full length TNFalpha. It can be used for pulldown assays. It binds TNFR1 and TNFR2 to induce downstream signaling pathways, but often requires higher concentrations than TNFalpha (catalog # U1120).

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified TNFalpha