Additional Information
| Product Name: | Anti-V5 |
|---|---|
| Also Known As: | Anti-V5 (V5.E10) Mouse Monoclonal Antibody |
| Catalog No.: | Y1081 |
| Size: | 100 µg |
| Molecular Weight: | ~55/25 kDa |
| Species: | N/A |
| Source: | Immunogen: GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH |
| Stock: | 10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide) |
| Concentration: | 1 µg/µl |
| Quality Assurance: | Guaranteed for detecting 1-2 ng of V5-tagged fusion proteins |
| Storage: | Store at -20°C. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | (Click image to enlarge)
 1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (lane 1) and transfected (lane 2) |
| Shipping Method: | Wet ice shipping |
| References: | N/A |
Details
Specificity
Recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins
Isotype
Mouse IgG1
Clone
V5.E10
Purification
Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor
Applications
Dot, ELISA, IP, IS, WB
Working Conditions
WB (with ECL): 1:1000-3000 dilution
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IS, etc), please determine optimal working dilution by titration test Images (Click image to enlarge)
1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (lane 1) and transfected (lane 2)
For best results with other assays (e.g.: Dot, ELISA, IS, etc), please determine optimal working dilution by titration test Images (Click image to enlarge)
1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (lane 1) and transfected (lane 2)
