Additional Information
| Product Name: | Anti-Ubiquitin (P4D1) |
|---|---|
| Also Known As: | Anti-Ub |
| Catalog No.: | Y3013 |
| Size: | 500 µg (5 X 100 µg) |
| Molecular Weight: | ~55/25 kDa |
| Species: | Recognizes ubiquitin in a wide range of species |
| Source: | Immunogen: Full length bovine ubiquitin |
| Stock: | 50 mM PIPES, Ph7.0, 1% sucrose, 0.5% dextran, 0.02% azide and 50% glycerol. |
| Concentration: | 1 mg/ml |
| Quality Assurance: | Guaranteed for detecting 5 ng endogenous ubiquitin or polyubiquitin chains in whole cell or tissue lysates at an antibody dilution of 1:1,000. |
| Storage: | Store at -20°C. Aliquot is not necessary. |
| PDF Data Sheet: | PDF Datasheet, MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | (Click image to enlarge)
 Figure 1.Western blot applications Anti-Ubiquitin (P4D1) was used at a 1:500 dilution. Bovine thymus ubiquitin was run as follows; Lane 1-50 ng, Lane 2-25 ng. Lane 3-12.5 ng, Lane 4-6.25 ng, Lane 5-3.12 ng. Lanes 6 & 7 represent 20 μg of Swiss 3T3 cell lysate from cells treated for 5h with 10 µM MG-132 (Lane 6) or untreated cells (Lane 7). Arrow indicates free ubiquitin band (8 kD), higher molecular weight bands are ubiquitinated proteins.  Figure 2. IF of HeLa cells HeLa cells were stained and visualized by fluorescence microscopy. Ubiquitin targeted cytoplasmic and nuclear proteins and free ubiquitin were stained using Anti-Ubiquitin (P4D1) at 1:500 dilution. |
| Shipping Method: | Wet ice shipping |
| References: | N/A |
Details
Specificity
Recognizes mono and polyubiquitinated proteins, free polyubiquitin chains and ubiquitin in a wide range of species. This antibody recognizes different types of polyubiqutin chains.
Isotype
Mouse IgG1
Clone
P4D1
Purification
Protein G affinity chromatography
Applications
WB and IF
Working Conditions
WB (with ECL): 1:1000 ~ 1:3000 dilution
IF: 1:500
IP: not recommended
For best results of WB and IF, and other assays (e.g.: Dot and ELISA), please determine optimal working dilution by titration test
Images
(Click image to enlarge)
Figure 1.Western blot applications Anti-Ubiquitin (P4D1) was used at a 1:500 dilution. Bovine thymus ubiquitin was run as follows; Lane 1-50 ng, Lane 2-25 ng. Lane 3-12.5 ng, Lane 4-6.25 ng, Lane 5-3.12 ng. Lanes 6 & 7 represent 20 μg of Swiss 3T3 cell lysate from cells treated for 5h with 10 µM MG-132 (Lane 6) or untreated cells (Lane 7). Arrow indicates free ubiquitin band (8 kD), higher molecular weight bands are ubiquitinated proteins.
Figure 2. IF of HeLa cells HeLa cells were stained and visualized by fluorescence microscopy. Ubiquitin targeted cytoplasmic and nuclear proteins and free ubiquitin were stained using Anti-Ubiquitin (P4D1) at 1:500 dilution.
Figure 1.Western blot applications Anti-Ubiquitin (P4D1) was used at a 1:500 dilution. Bovine thymus ubiquitin was run as follows; Lane 1-50 ng, Lane 2-25 ng. Lane 3-12.5 ng, Lane 4-6.25 ng, Lane 5-3.12 ng. Lanes 6 & 7 represent 20 μg of Swiss 3T3 cell lysate from cells treated for 5h with 10 µM MG-132 (Lane 6) or untreated cells (Lane 7). Arrow indicates free ubiquitin band (8 kD), higher molecular weight bands are ubiquitinated proteins.
Figure 2. IF of HeLa cells HeLa cells were stained and visualized by fluorescence microscopy. Ubiquitin targeted cytoplasmic and nuclear proteins and free ubiquitin were stained using Anti-Ubiquitin (P4D1) at 1:500 dilution.
