Anti-HA

Anti-HA (HA.C5) Mouse Monoclonal Antibody

Additional Information

Product Name: Anti-HA
Also Known As: Anti-HA (HA.C5) Mouse Monoclonal Antibody
Catalog No.: Y1071
Size: 100 µg
Molecular Weight: ~55/25 kDa
Species: N/A
Source: Immunogen: YPYDVPDYA (HA) synthetic peptide conjugated to KLH
Stock: 10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide)
Concentration: 1 µg/µl
Quality Assurance: Guaranteed for detecting 1-5 ng of HA-tagged fusion proteins
Storage: Store at -20°C. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles.
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)


1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (lane 1) and transfected (lane 2)
Shipping Method: Wet ice shipping
References: N/A

Details

Specificity
Recognizes the N-terminal, C-terminal or internal HA- tagged fusion proteins

Isotype
Mouse IgG3

Clone
HA.C5

Purification
Protein A affinity chromatography from mouse ascites fluid

Applications
Dot. ELISA, IP, IS, WB

Working Conditions
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IS, etc), please determine optimal working dilution by titration test

Images
(Click image to enlarge)


1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (lane 1) and transfected (lane 2)