Anti-GFP

Anti-GFP (GF28R) Mouse Monoclonal Antibody

Additional Information

Product Name: Anti-GFP
Also Known As: Anti-GFP (GF28R) Mouse Monoclonal Antibody
Catalog No.: Y1030
Size: 25 µg
Molecular Weight: ~55/25 kDa
Species: N/A
Source: Immunogen: GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates
Stock: 10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide)
Concentration: 1 µg/µl
Quality Assurance: WB (with ECL): 1-10 ng of purified GFP or GFP fusion proteins
Storage: Store at -20°C. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles.
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)


1:1000 (1 μg/ml) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (Lane 1), 1μg (Lane 2) and 10μg (Lane 3) of cell lysates used
Shipping Method: Wet ice shipping
References: N/A

Details

Specificity
Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP

Isotype
Mouse IgG2b

Clone
GF28R

Purification
Protein A affinity chromatography from mouse ascites fluid

Applications
Dot, ELISA, IP, IS, WB

Working Conditions
WB (with ECL): 1:1000-3000 dilution
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Images
(Click image to enlarge)


1:1000 (1 μg/ml) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (Lane 1), 1μg (Lane 2) and 10μg (Lane 3) of cell lysates used