Anti-GAPDH

Anti-GAPDH (GA1R) Loading Control Mouse Monoclonal Antibody

Additional Information

Product Name: Anti-GAPDH
Also Known As: Anti-GAPDH (GA1R) Loading Control Mouse Monoclonal Antibody
Catalog No.: Y1040
Size: 25 µg
Molecular Weight: ~55/25 kDa
Species: Human
Source: Immunogen: Recombinant GAPDH
Stock: 10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide)
Concentration: 1 µg/µl
Quality Assurance: Guaranteed for detecting endogenous GPADH in 20 µg cell or tissue lysates
Storage: Store at -20°C. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles.
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)


Figure 1. Three loading control mAbs reacting against 10 μg/lane of mouse brain tissue lysates. 50 kDa band is Anti-β-Tubulin (BT7R) at 1:2000 dilution (0.5 μg/ml); 42 kDa band is Anti-β-Actin (BA3R) at 1:1000 dilution (1 μg/ml); 37 kDa band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2 μg/ml)


Figure 2. Left: 1:2000 (0.5μg/mL) Ab dilution used in WB of 5μg/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). Right: WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3)
Shipping Method: Wet ice shipping
References: N/A

Details

Specificity
Recognizes native and denatured forms of GAPDH (~37kDa)

Isotype
Mouse IgG1

Clone
GA1R

Purification
Protein A affinity chromatography from mouse ascites fluid

Applications
Dot, ELISA, IS, WB

Cross Reactivity
GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken, and hamster. GAPDH from other species may also be detectable

Working Conditions
WB (with ECL): 1:1000-100,000 dilution
For best results with other assays (e.g.: Dot, ELISA, IS, etc), please determine optimal working dilution by titration test

Images
(Click image to enlarge)


Figure 1. Three loading control mAbs reacting against 10 μg/lane of mouse brain tissue lysates. 50 kDa band is Anti-β-Tubulin (BT7R) at 1:2000 dilution (0.5 μg/ml); 42 kDa band is Anti-β-Actin (BA3R) at 1:1000 dilution (1 μg/ml); 37 kDa band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2 μg/ml)


Figure 2. Left: 1:2000 (0.5μg/mL) Ab dilution used in WB of 5μg/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). Right: WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3)