Anti-DYKDDDDK (FLAG)

Anti-DYKDDDDK (FLAG) (FG4R) Mouse Monoclonal Antibody

Additional Information

Product Name: Anti-DYKDDDDK (FLAG)
Also Known As: Anti-DYKDDDDK (FLAG) (FG4R) Mouse Monoclonal Antibody
Catalog No.: Y1101
Size: 100 µg
Molecular Weight: ~55/25 kDa
Species: N/A
Source: Immunogen: DYKDDDDK (FLAG) synthetic peptide conjugated to KLH
Stock: 10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide)
Concentration: 1 µg/µl
Quality Assurance: Guaranteed for detecting 1-5 ng of DYKDDDDK-tagged fusion proteins
Storage: Store at -20°C. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles.
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)


1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (lane 1) and transfected (lane 2)
Shipping Method: Wet ice shipping
References: N/A

Details

Specificity
Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins

Isotype
Mouse IgG1

Clone
FG4R

Purification
Protein A affinity chromatography from mouse ascites fluid

Applications
Dot, ELISA, IP, IS, WB

Working Conditions
WB (with ECL): 1:1000-3000 dilution
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IS, etc), please determine optimal working dilution by titration test

Images
(Click image to enlarge)


1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (lane 1) and transfected (lane 2)