Anti-beta-Actin (Rabbit mAb)

This is a rabbit monoclonal anti-beta-actin antibody. β-actin (molecular weight 42 kDa) is a member of highly conserved actins that are involved in cell motility, structure, and integrity. It is commonly used as a loading control in immunoblotting.

Additional Information

Product Name: Anti-beta-Actin (Rabbit mAb)
Also Known As: Anti-β-Actin Rabbit Monoclonal Antibody
Catalog No.: Y1055
Size: 100 µl
Molecular Weight: ~55/25 kDa
Species: Human
Source: Immunogen: full length human beta-actin protein.
Stock: 10 mM PBS (pH 7.2), 50% Glycerol, 0.09% NaN3 (sodium azide)
Concentration: ~2-3 µg/µl depending on batches
Quality Assurance: Guaranteed for detecting endogenous β-Actin in 20 µg cell or tissue lysates at 1:10,000 antibody dilution.
Storage: Store at -20°C. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles.
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s):
Shipping Method: Wet ice shipping
References: N/A

Details

Specificity
Recognizes β-Actin (~42kDa)

Isotype
Rabbit IgG

Clone
4C4

Purification
Protein A affinity chromatography

Applications
ELISA, IS, WB

Cross Reactivity
β-Actin from human, mouse, rat, rabbit, pig and yeast. β-Actin from other species may also be detectable

Working Conditions
WB (with ECL): 1:3,000-10,000 dilution
For best results, please determine optimal working dilution by titration test