Anti-6xHis

Anti-His (HIS.H8) Mouse Monoclonal Antibody

Additional Information

Product Name: Anti-6xHis
Also Known As: No
Catalog No.: Y1010
Size: 25 µg
Molecular Weight: ~55/25 kDa
Species: N/A
Source: Immunogen: HHHHHH (6x His) synthetic peptide conjugated to KLH
Stock: 10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide)
Concentration: 1 µg/µl
Quality Assurance: WB (with ECL): 1-2 ng of His-tagged fusion proteins
Storage: Store at -20°C. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles.
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)


Figure 1. Left: untransfected control; Right: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)


Figure 2. Comparison between anti-His tag (HIS.H8) mAb with two different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5 μg/ml)


Figure 3. Standard ladder containing five different His-tagged proteins; untransfected control (Lane 1), HEK293 cells transfected with His-tagged protein vector (Lane 2)
Shipping Method: Wet ice shipping
References: N/A

Details

Specificity
Recognizes the epitope of 6xHis-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6xHis-tags)

Isotype
Mouse IgG2b

Clone
HIS.H8

Purification
Protein A affinity chromatography from mouse ascites fluid

Applications
Dot, ELISA, IP, IS, WB

Working Conditions
WB (with ECL): 1:1000-3000
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Images
(Click image to enlarge)


Figure 1. Left: untransfected control; Right: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)


Figure 2. Comparison between anti-His tag (HIS.H8) mAb with two different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5 μg/ml)


Figure 3. Standard ladder containing five different His-tagged proteins; untransfected control (Lane 1), HEK293 cells transfected with His-tagged protein vector (Lane 2)