GST-USP30(57-517)

USP30(57-517) contains the catalytic domain of USP30 with the N-terminal membrane bound domain being deleted. USP30 deubiquitinates substrates of Parkin including MIRO1, TOMM20, MFN1, and MFN2 on mitochondrial membranes and functions to suppress mitophagy. It prefers cleavage of K6- and K11-linked polyubiquitin chains.

Additional Information

Product Name: GST-USP30(57-517)
Also Known As: N/A
Catalog No.: H7810
Size: 50 µg
Molecular Weight: 51.5 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~ 70% by SDS-PAGE
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_032663.4
Image(s): Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified GST-USP30(57-517)
Shipping Method: Dry ice shipping
References: 1. Bingol B, et al. (2014) Nature 510: 370
2. Cunningham CN, et al. (2015) Nat Cell Biol. 17: 160
3. Wang, Y, et al. (2015) Autophagy 11: 595

Details

USP30(57-517) contains the catalytic domain of USP30 with the N-terminal membrane bound domain being deleted. USP30 deubiquitinates substrates of Parkin including MIRO1, TOMM20, MFN1, and MFN2 on mitochondrial membranes and functions to suppress mitophagy. It prefers cleavage of K6- and K11-linked polyubiquitin chains.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified OTUD6B