Product Image(s)
[Cat.# F1200, F1201, F1202] PS-341
| 100 nM bovine 26S proteasome (Cat. # A1200) was incubated with DMSO or with 10 µM PS-341 for 10 min at 37 °C in 20 mM Tris, pH 7.1, 50 mM NaCl, 2 mM ATP, 5 mM MgCl2, 2 mM bME and 10% glycerol. The proteasome was then diluted 10X into 50 µM SUC-LLVY-AMC (Cat. # G1100) in a buffer containing 20 mM Tris, pH 7.1, 2 mM bME. The released AMC fluorescence was monitored by a plate reader. |
| Equal amount of whole cell lysates prepared from DMSO (lane 1) or 10 μM PS-341 (lane 2)-treated HeLa cells were separated by SDS-PAGE and immunoblotted with an anti-Ub antibody. Cells were treated with DMSO or PS-341 for 16 hours. |
